Technical Data
Granulocyte Macrophage Colony Stimulating Factor, Recombinant, Mouse (GM-CSF, Burst Promoting Activity, CMCSF, Colony Stimulating Factor 2, CSF Alpha, Eosinophil Colony Stimulating Factor, Granulocyte Macrophage Colony Stimulating Factor, Molgramostin, Pl
Molecular Biology Storage: -20CShipping: RT
Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) is a potent species specific stimulator of bone marrow cells. GM-CSF stimulates precursor cells of granulocytes, macrophages, and eosinophils. Murine GM-CSF is a 14.0kD protein containing 125 amino acid residues.

Biological Activity: Determined by the dose-dependent stimulation of the proliferation of murine FDC-P1 cells. The ED50 is 0.2ng/ml, corresponding to a specific activity of 5x10e6u/mg.

Responding Cells (partial list): Certain BM Precursor cells. For most in vitro applications, murine GM-CSF exerts its biological activity in the concentration range of 0.05-0.5ng/ml.

Amino Acid Sequence: The sequence of the first five N-terminal amino acids was found to be Met-Ala-Pro-Thr-Arg.

Storage and Stability:
For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Country of Origin: USA

Molecular Weight:
Source: Murine, recombinant protein expressed in E. coli
Purity: 98% by SDS-PAGE and HPLC. Endotoxin; 0.1ng/ug (1EU/ug)
Concentration: ~0.1mg/ml
Form: Supplied as a liquid in 20mM acetic acid. Dilute to working concentration with dH2O or aqueous buffers, 0.1% HSA or BSA.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.