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Technical Data |
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G8950-20A |
Granulocyte Macrophage Colony Stimulating Factor, Recombinant, Mouse (GM-CSF, Burst Promoting Activity, CMCSF, Colony Stimulating Factor 2, CSF Alpha, Eosinophil Colony Stimulating Factor, Granulocyte Macrophage Colony Stimulating Factor, Molgramostin, Pl |
2ug 10ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: RT |
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Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) is a potent species specific stimulator of bone marrow cells. GM-CSF stimulates precursor cells of granulocytes, macrophages, and eosinophils. Murine GM-CSF is a 14.0kD protein containing 125 amino acid residues. Biological Activity: Determined by the dose-dependent stimulation of the proliferation of murine FDC-P1 cells. The ED50 is 0.2ng/ml, corresponding to a specific activity of 5x10e6u/mg. Responding Cells (partial list): Certain BM Precursor cells. For most in vitro applications, murine GM-CSF exerts its biological activity in the concentration range of 0.05-0.5ng/ml. Amino Acid Sequence: The sequence of the first five N-terminal amino acids was found to be Met-Ala-Pro-Thr-Arg. Storage and Stability: For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Country of Origin: USA Molecular Weight: 14.2kD |
Source: Murine, recombinant protein expressed in E. coli Purity: 98% by SDS-PAGE and HPLC. Endotoxin; 0.1ng/ug (1EU/ug) Concentration: ~0.1mg/ml Form: Supplied as a liquid in 20mM acetic acid. Dilute to working concentration with dH2O or aqueous buffers, 0.1% HSA or BSA. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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