Technical Data
G8950-33
Granulocyte Macrophage Colony Stimulating Factor, Recombinant, Human (GM-CSF, Burst Promoting Activity, CMCSF, Colony Stimulating Factor 2, CSF Alpha, Eosinophil Colony Stimulating Factor, Granulocyte Macrophage Colony Stimulating Factor, Molgramostin, Pl
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
Human GM-CSF is a 14.6kD globular protein consisting of 128 amino acid containing two dislufied bonds. GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells.

Recombinant human GM-CSF was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Molecular Weight: 14.6kD (128 amino acids)

Sequence:
MAPARSPSPS/ TQPWEHVNAI/ QEARRLLNLS/ RDTAAEMNET/ VEVISEMFDL/ QEPTCLQTRL/ ELYKQGLRGS/ LTKLKGPLTM/ MASHYKQHCP/ PTPETSCATQ/ IITFESFKEN/ LKDFLLVIPF/ DCWEPVQE

Endotoxin:<1EU/1ug of protein (LAL)

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
14.6 kD
Source: Recombinant, Human, E. coli
Purity: 95% by SDS PAGE
Concentration: ~1mg/ml
Form: Supplied as a liquid in PBS, pH 7.4

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Trapnell BC, et al. (2002) Annu Rev Physiol. 64, 775-802 2. Wong GG, et al (1985) Science, 228, 810-815

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.