Technical Data
Granulocyte Colony Stimulating Factor, 127aa, Recombinant, Human (G-CSF, Filgrastim, Lenograstim, Pluripoietin)
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant GM-CSF comprises a 127 amino acid fragment (18-144) corresponding to the mature GM-CSF protein and is expressed in E. coli with an amino-terminal hexahistidine tag.

GM-CSF is a potent species-specific growth factor produced by a variety of cell types including T cells, B cells, macrophages, mast cells and endothelial cells. GM-CSF is produced in response to cytokine or immune stimulation and has been shown to stimulate the proliferation, maturation and function of hematopoietic cells.

Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: Human, Recombinant, E. coli
Purity: Purified. 95% by SDS-PAGE.
Concentration: As reported

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Burgess, A.W. et al. (1987) Blood 69 (1) : 43-51
Metcalf, D et al. (1986) Blood 67 (1) : 37-45.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.