Technical Data
GM-CSF/IL-3 Fusion Protein, Recombinant, Human (PIXY321)
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant Human GMCSF/IL3 fusion protein produced in E.Coli is a single, non-glycosylated, polypeptide chain having a Mw of 30kD. Synergism of IL3 together with GMCSF causes a rapid rise in neutrophils due to GMCSF and a slower rise in platelets due to IL3 which has strong stimulated effect on hematopoietic cells. The analog of GMCSF/IL3 is PIXY321 (Pixykine). Results indicate that PIXY321 can stimulate multilineage hematopoiesis in vivo and enhance neutrophil and platelet recovery following chemotherapy and bone marrow transplantation (BMT). These results suggest that PIXY321 elicits the biological effects of both its component cytokines and represents a novel means of delivering two independent but interactive cytokines in combination.

Biological Activity:
Recombinant Human GMCSF/IL-3 FP is fully biologically active when compared to standard.
The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is 0.2ng/ml, corresponding to a Specific Activity of 5x10e6 IU/mg.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel. Purified by proprietary chromatographic techniques.
Form: Supplied as a lyophilized powder from PBS, pH 7.4 , 0.1% BSA. Reconstitute with sterile ddH2O to 0.1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.