		Technical Data
G8959-50	GRASP (GRIP associated protein 1, GRIP1 associated protein 1, GRIPAP1, KIAA1167)

Description:
PDZ domain-containing proteins, such as PSD-95 and GRIP are thought to play key roles in glutamate receptor plasticity. GRIP-associated proteins (GRASPs) that bind to distinct PDZ domains within GRIP also play key roles in regulation of glutamate receptor function. GRASP-1 is a neuronal rasGEF associated with GRIP and AMPA receptors in vivo. Recent work suggests that GRASP-1 may regulate neuronal ras signaling and contribute to the regulation of AMPA receptor distribution by NMDA receptor activity.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

As reported

Immunogen:

Peptide corresponding to amino acid residues from the C-terminal region of rat GRASP, conjugated to keyhole limpet hemocyanin (KLH).

Purity:

Purified by immunoaffinity chromatography.

Form

10 mM Hepes (pH7.5), 150 mM NaCl, 100 ug/ml BSA and 50% glycerol

Specificity:

Specific for the ~95k GRASP protein. Species Crossreactivity: Human, mouse, and rat based on 100% antigen sequence homology.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Scannevin RH, Huganir RL (2000) Postsynaptic organization and regulation of excitatory synapses. Nat Rev Neurosci 1:133-141.
2. Ye B, Liao DZ, Zhang XQ, Zhang PS, Dong HL, Huganir RL (2000) GRASP-1: A neuronal RasGEF associated with the AMPA receptor/GRIP complex. Neuron 26:603-617.