		Technical Data
G8965-01	Green Fluorescent Protein (GFP)

Description:
GFP is a 27kD monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (395nm) and emits green light (508nm) in the absence of additional proteins, substrates or co-factors. Intact, native GFP protein is required for fluorescence. The GFP chromophore results from the cyclization and oxidation of the sequence Ser65-Tyr66-Gly67. GFP fluorescence is stable, species independent and suitable for a variety of applications.

Applications:
Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
ELISA: 1:1000-1:10,000
Immunohistochemistry: 1:1000.
Mouse brain neural cells (1:1000) RT, overnight using secondary antibody anti-rat IgG-Cy3 (1:300) RT, 1hr. Data courtesy of Yoshihiro Yoshyihara RIKEN Brain Science Institute.
Optimal dilutions to be determined by the researcher.

Hybridoma: Mouse GF200

Source: Ascites

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1,k	GF200	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		4°C (-20°C Glycerol)	Blue Ice		Mouse

Concentration:

~1mg/ml

Immunogen:

Purified recombinant His-GFP full length fusion protein (Aequorea victoria).

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.02% sodium azide before the addition of glycerol to 40%.

Specificity:

Reacts with Green Fluorescent Protein (GFP) and crossreacts with Blue Fluorescent Protein (BFP).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Prasher, D.C., et al. (1992) Gene 111:229-233 (1982). 2. Chalfie, M., et al. (1994) Science 263:802-805 (1994). 3. Youvan, D.C. (1995) Science 268:264 (1995). 4. Ilic, D., et al; J. Cell Biol. 143(2):547-560 (1998).