Technical Data
G8965-01
Green Fluorescent Protein (GFP)
Description:
GFP is a 27kD monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (395nm) and emits green light (508nm) in the absence of additional proteins, substrates or co-factors. Intact, native GFP protein is required for fluorescence. The GFP chromophore results from the cyclization and oxidation of the sequence Ser65-Tyr66-Gly67. GFP fluorescence is stable, species independent and suitable for a variety of applications.

Applications:
Suitable for use in Western Blot and ELISA. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:1000-1:2000
ELISA: 1:2000-1:20,000
Optimal dilutions to be determined by the researcher.

Hybridoma: Mouse GF200

Source: Ascites

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,kGF200Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Purified recombinant His-GFP full length fusion protein (Aequorea victoria).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide before the addition of glycerol to 40%.
Specificity:
Reacts with Green Fluorescent Protein (GFP) and crossreacts with Blue Fluorescent Protein (BFP).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Prasher, D.C., et al. (1992) Gene 111:229-233 (1982). 2. Chalfie, M., et al. (1994) Science 263:802-805 (1994). 3. Youvan, D.C. (1995) Science 268:264 (1995). 4. Ilic, D., et al; J. Cell Biol. 143(2):547-560 (1998).