Technical Data
G8965-05A
Green Fluorescent Protein, aa1-238, Recombinant, Aequorea victoria (GFP)
100ug
BioSeparation? Storage: -20°CShipping: Blue Ice
GFP, also known as green fluorescent protein, is a protein produced by the jellyfish (Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.

Source: Recombinant GFP protein, expressed in E.coli.

Molecular Weight: 26.8kD (238aa)

Endotoxin Level: 1.0 EU /1ug of protein (LAL).

AA Sequence:
MSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTFSYGVQC FSRYPDHMKQ HDFFKSAMPE GYVQERTIFF KDDGNYKTRA EVKFEGDTLV NRIELKGIDF KEDGNILGHK LEYNYNSHNV YIMADKQKNG IKVNFKIRHN IEDGSVQLAD
HYQQNTPIGD GPVLLPDNHY LSTQSALSKD PNEKRDHMVL LEFVTAAGIT HGMDELYK

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
26.8kD
Source: E. coli
Purity: 95% by SDS-PAGE
Concentration: ~1mg/ml
Form: Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, 10% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Millán MI, et al. (2009). Medicina. 69(3):370-4, 2. Marshall J, et al. (1995). Neuron, 14(2):211-5.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.