![]() |
Technical Data |
|
G8965-05A |
Green fluorescent protein, aa1-238, Recombinant, Aequorea victoria (GFP) |
100ug |
| BioSeparation? | Storage: -20°CShipping: Blue Ice |
|
GFP, also known as green fluorescent protein, is a protein produced by the jellyfish (Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Source: Recombinant GFP protein, expressed in E.coli. Molecular Weight: 26.8kD (238aa) Endotoxin Level: 1.0 EU /1ug of protein (LAL). AA Sequence: MSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTFSYGVQC FSRYPDHMKQ HDFFKSAMPE GYVQERTIFF KDDGNYKTRA EVKFEGDTLV NRIELKGIDF KEDGNILGHK LEYNYNSHNV YIMADKQKNG IKVNFKIRHN IEDGSVQLAD HYQQNTPIGD GPVLLPDNHY LSTQSALSKD PNEKRDHMVL LEFVTAAGIT HGMDELYK Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 26.8kD |
Source: E. coli Purity: 95% by SDS-PAGE Concentration: ~1mg/ml Form: Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, 10% glycerol. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
|
1. Millán MI, et al. (2009). Medicina. 69(3):370-4, 2. Marshall J, et al. (1995). Neuron, 14(2):211-5.
|
||