Technical Data
G8965-07
Green Fluorescent Protein (GFP)
Description:
The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27kD monomer consisting of 238 amino acids and emits green light (emission maximum at 509nm) when excited with blue or UV light.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:100,000
Western Blot: 1:1500-1:15,000. Use either AP or HRP conjugated antibodies.
Immunohistochemistry:1:500-1:2500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k1001Affinity Purified
SizeStorageShippingSourceHost
500ug4C (-20C Glycerol)Blue IceMouse
Concentration:
~1mg/ml
Immunogen:
GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jelly sh Aequorea victoria.
Purity:
Purified by Protein A affinity chrmoatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognized wild type and recombinant forms of GFP. No reaction is seen against RFP. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti mouse serum and purified and partially purified green fluorescent protein (Aequorea victoria).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.