Technical Data
G8965-08
Green Fluorescent Protein (GFP)
Description:
The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27kD monomer consisting of 238 amino acids and emits green light (emission maximum at 509nm) when excited with blue or UV light.

Applications:
Suitable for use in Immunocytochemistry, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilution to be determined by researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG12Q2156Purified
SizeStorageShippingSourceHost
100ug4°C (-20°C Glycerol)Blue IceMouse
Concentration:
~0.5mg/ml
Immunogen:
Bacterial-expressed GFP fusion protein.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes natural GFP. Directed against a bacterial-expressed GFP fusion protein. Reactivity to eGFP or other mutant varieties is poor to non-existent.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Lei, Q., et al., PNAS (2000) 97:9771–9776.