Technical Data
Green Fluorescent Protein (GFP)
The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights into the physiological activities of living cells.

Suitable for use in ELISA, Electron Microscopy, Western Blot, Immunoprecipitation, Flow Cytometry, Immunofluorescence/Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000-1:40,000
Western Blot: 1:1000-1:3000
Immunofluorescence (IC): 1:10-1:500
Immunohistochemistry: 1:10-1:500
Immunohistochemistry (Paraffin): 1:10-1:500
Immunoprecipitation: 1:10-1:500
Optimal dilution to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
50ul-20CBlue IceRabbit
Highly purified recombinant (full length) GFP made in E. coli.
Purified by Immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Recognizes Green Fluorescent Protein (GFP). Species crossreactivity: all variants of Aequorea victoria GFP; S65T-GFP, RS-GFP, YFP and EGFP.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.McCabe, et al. Functional roles for fatty Acylated Amino-terminal domains in subcellular localization. Mol Biol Cell. 10: 3771-3786, 1999. 2.Jasinska, et al. Lipid phosphate phosphohydrolase-1 degrades exogenous glycerolipid and sphingolipid phosphate esters J Biochem. 340: 677-686, 1999. 3. .Zhang, et al. Identification of structurally important domains of lipid phosphate phosphatase-1: implications for sites of action J Biochem. 345: 181-184, 2000. 4.Mesaeli, N., et al. Calreticulin is essential for cardiac development. J. Cell Biology. 144(5): 857-68, 1999.