Technical Data
G8965-10A
Green Fluorescent Protein (GFP)
Description:
The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights into the physiological activities of living cells.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Western Blot: Detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins.
Immunocytochemistry: Very good signal on recombinant YES-GFP chimeras expressed in COS cells.
Immunohistochemistry: 1:200 with HRP detection systems. Has been shown to be useful on mouse sections fixed with formalin and on whole-mount mouse embryos.
Optimal dilution to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ul-20CBlue IceRabbit
Concentration:
~1mg/ml
Immunogen:
Highly purified recombinant (full length) GFP made in E. coli.
Purity:
Purified by affinity chromatography.Product Type: Pab Isotype: IgG Host: rabbit Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. Purity: Purified by affinity chromatography. Immunogen: Highly purified recombinant (ful
Form
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity:
Recognizes Green Fluorescent Protein (GFP). Species crossreactivity: all variants of Aequorea victoria GFP; S65T-GFP, RS-GFP, YFP and EGFP. Detects 5ng of recombinant GFP (ECL) with 1 minute exposure or less.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.McCabe, et al. Functional roles for fatty Acylated Amino-terminal domains in subcellular localization. Mol Biol Cell. 10: 3771-3786, 1999. 2.Jasinska, et al. Lipid phosphate phosphohydrolase-1 degrades exogenous glycerolipid and sphingolipid phosphate esters J Biochem. 340: 677-686, 1999. 3. .Zhang, et al. Identification of structurally important domains of lipid phosphate phosphatase-1: implications for sites of action J Biochem. 345: 181-184, 2000. 4.Mesaeli, N., et al. Calreticulin is essential for cardiac development. J. Cell Biology. 144(5): 857-68, 1999.