Technical Data
G8965-12
GFP (Green Fluorescent Protein)
Description:
Green fluorescent protein (GFP) is a spontaneously fluorescent protein isolated from the Pacific jellyfish, Aequorea victoria. It transduces the blue chemiluminescence into green fluorescent light. Since the molecular cloning of GFP cDNA and the demonstration that GFP can be expressed as a functional transgene, GFP has become a unique new tool with exciting applications in cell, developmental and molecular biology. GFP is an ideal fluorescent probe: its fluorescence is not species specific (it has been expressed from bacteria, yeast, plant to mammalian cells); it can fuse with proteins of interest without interfering significantly with their assembly or function.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:5000
Immunoprecipitation: 1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRabbit
Concentration:
~1mg/ml
Immunogen:
E. coli-expressed full length GFP (green fluorescent protein).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity:
Recognizes wild-type GFP and its variants such as EGFP and EBFP.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Muller, O. et al, J. Cell Science 116: 1107-1115 (2003). 2. Eldstrom, J. et al., FEBS Letts. 531: 529-537 (2002). 3. Brown, D.D. et al., PNAS 102: 12455-12458 (2005). 4. Tulin, A. et al, Genetics 172; 363-371 (2006).