Technical Data
GRO beta, Recombinant, Human (CXCL2, MIP-2, Macrophage Inflammatory Protein 2)
Molecular Biology Storage: -20CShipping: RT
Recombinant Human GRO-beta is a single,non-glycosylated, polypeptide chain containing 73 amino acids and having a molecular mass of 7908 Dalton. Human GRO-beta is purified by proprietary chromatographic techniques.

Sequence :
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Leu-Ala-Thr.

Dimers and Aggregates:
1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:
GRO-beta is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract CXCR2 transfected 293 cells using 10.0-100.0ng/ml.

0.1ng/ug (IEU/ug) of Human GRO-beta.

Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm.
2. Analysis by RP-HPLC, using a calibrated solution of Human GRO-beta as a Reference Standard.

Reconstitute the lyophilized Human GRO-beta in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Source: E. coli
Purity: 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Form: Supplied as a lyophilized powder. No additives.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Modulation of the neurotransmitter release in rat cerebellar neurons by GRO beta. Ragozzino D, Giovannelli A, Limatola C, Neuroreport 1998 Nov 16;9(16):3601-6 2. gro-beta, a -C-X-C- chemokine, is an angiogenesis inhibitor that suppresses the growth of Lewis lung carcinoma in mice. Cao Y, Chen C, Tsang M, J Exp Med 1995 Dec 1;182(6):2069-77 3. Expression of active human GRO beta and GRO gamma neutrophil chemotactic proteins in E. coli. Zagorski J, DeLarco JE, Protein Expr Purif 1994 Aug;5(4):337-45 4. Differential expression of the small inducible cytokines GRO alpha and GRO beta by synovial fibroblasts in chronic arthritis: possible role in growth regulation. Hogan M, Sherry B, Fabre M, Cytokine 1994 Jan;6(1):61-9 5. Interleukin-1-inducible expression of gro-beta via NF-kappa B activation is dependent upon tyrosine kinase signaling. Joshi-Barve SS, Rangnekar VV, Rangnekar VM, J Biol Chem 1993 Aug 25;268(24):18018-29 6. The interleukin-8-related chemotactic cytokines GRO alpha, GRO beta, and GRO gamma activate human neutrophil and basophil leukocytes. Geiser T, Dewald B, Clark-Lewis I, J Biol Chem 1993 Jul 25;268(21):15419-24.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.