Technical Data
G8975-25
GRO gamma, Recombinant, Human
2ug
10ug
Molecular Biology Storage: -20°CShipping: Blue Ice
CXCL3/GROg is a small cytokine belonging to the CXC chemokine family. CXCL3 controls migration and adhesion of monocytes and mediates it effects on its target cell by interacting with a cell surface chemokine receptor called CXCR2. The three GRO cDNAs encode 107 amino acid precursor proteins from which the N-terminal 34 amino acid residues are cleaved to generate the mature GROs. There are no potential N-linked glycosylation sites in the amino acid sequences. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively.Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B.

GRO gamma Recombinant, Human is a 7.9kD protein containing 73 amino acid residues.

Source: E. coli

Form: Lyophilized protein without additives.

Stability: The lyophilized protein, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted rat GRO- should be stored in working aliquots at -20°C.

Purity: 98% by SDS-PAGE and HPLC analyses.

Endotoxin: 0.1ng/ug.

Reconstitution: Soluble in water and most aqueous buffers. The lyophilized protein should be reconstituted in water to a concentration of 100ng/ul. This solution can be diluted into water or other buffered solutions or stored at -20°C for future use.

Biological Activity: Maximal chemotactic activity on rat neutrophils is assayed in a modified Boyden chamber at 50ng/ml.

Responding Cells (partial list): Neutrophils

Concentration Range for most in vitro applications: 10-100ng/ml

Country of Origin: USA

Molecular Weight:
7.9kD
Source: Human

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.