Technical Data
G8975-25A
GRO gamma, Recombinant, Human (Chemokine, C-X-C Motif Ligand 3, CXCL3, CINC-2b, GRO3, GROg, Growth-regulated Protein gamma, Macrophage Inflammatory Protein 2-beta Precursor, MGSA gamma, MIP-2b, MIP2B, SCYB3)
2ug
10ug
Growth Factors, Cytokines Storage: -20CShipping: RT
CXCL3/GROg is a small cytokine belonging to the CXC chemokine family. CXCL3 controls migration and adhesion of monocytes and mediates it effects on its target cell by interacting with a cell surface chemokine receptor called CXCR2. The three GRO cDNAs encode 107 amino acid precursor proteins from which the N-terminal 34 amino acid residues are cleaved to generate the mature GROs. There are no potential N-linked glycosylation sites in the amino acid sequences. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively.Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B.

GRO gamma Recombinant, Human is a 7.9kD protein containing 73 amino acid residues.

Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Ser-Val-Val-Thr.

Dimers and Aggregates:
1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:
GRO-gamma is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract CXCR2 transfected 293 cells using 10.0-100.0 ng/ml.

Endotoxin:
0.1ng/ug (IEU/ug) of Human GRO-gamma.

Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm.
2. Analysis by RP-HPLC, using a calibrated solution of Human GRO-gamma as a Reference Standard.

Reconstitution:
Reconstitute the lyophilized Human GRO-gamma in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Source: E. coli
Purity: 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Concentration: ~0.1mg/ml
Form: Supplied as a lyophilized powder. No additives.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Isolation of the CXC chemokines ENA-78, GRO alpha and GRO gamma from tumor cells and leukocytes reveals NH2-terminal heterogeneity. Functional comparison of different natural isoforms. Wuyts A, Govaerts C, Lenaerts JP, Van Damme J, Eur J Biochem 1999 Mar;260(2):421-9 2. Expression of active human GRO beta and GRO gamma neutrophil chemotactic proteins in E. coli. Zagorski J, DeLarco JE, Protein Expr Purif 1994 Aug;5(4):337-45 3. The interleukin-8-related chemotactic cytokines GRO alpha, GRO beta, and GRO gamma activate human neutrophil and basophil leukocytes. Geiser T, Dewald B, Clark-Lewis I, J Biol Chem 1993 Jul 25;268(21):15419-24.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.