Technical Data
Growth and Differentiation Factor 5, Recombinant, Human (GDF5)
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant human GDF-5 produced in E. coli is a homodimer comprised of a non-glycosylated polypeptide chain containing 2 x 117 amino acids and having a total molecular mass of 26.8kD. To enable bacterial expression of rhGDF-5 we removed the N-terminal sequence Ala-Pro-Leu-Thr and added the Lys. GDF5 is purified by proprietary chromatographic techniques.

Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Lys-Arg-Gln-Gly.

Biological Activity:
Human GDF-5 is fully biologically active when compared to standard. Induction of alkaline phosphatase acrtivity in ATDC5 cells: EC50 = 40Nm.

Less than 0.1ng/ug (IEU/ug) of GDF-5.

Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.15 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of GDF-5 as a Reference Standard.

Storage and Stability:
Lyophilized powder is stable for at least 12 months at -20C Reconstituted product must be stored in aliquots at -20C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE Silver Stained gel. Endotoxin: 0.1ng/ug (IEU/ug).
Form: Supplied as a lyophilized powder without any additives. Reconstitute with sterile 20mM AcOH to 100ug/ml, which can then be further diluted to other aqueous solutions with 0.1% BSA or HSA.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Spine 31(25): 2909-2917 (2006). 2. Connect Tissue Res. 47(5): 264-270 (2006). 3. J. Anat. 201(5): 425 (2002). 4. J. Anat. 200(2): 213 (2002). 5. J. Anat. 200(2): 213 (2002). 6. J. Anat. 200(2): 212 (2002).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.