Technical Data
G8989-45
Growth and Differentiation Factor 5, Recombinant, Human (GDF5)
10ug
50ug
50ug
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Technical Data |
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G8989-45 |
Growth and Differentiation Factor 5, Recombinant, Human (GDF5) |
10ug 50ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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Recombinant human GDF-5 produced in E. coli is a homodimer comprised of a non-glycosylated polypeptide chain containing 2 x 117 amino acids and having a total molecular mass of 26.8kD. To enable bacterial expression of rhGDF-5 we removed the N-terminal sequence Ala-Pro-Leu-Thr and added the Lys. GDF5 is purified by proprietary chromatographic techniques. Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Lys-Arg-Gln-Gly. Biological Activity: Human GDF-5 is fully biologically active when compared to standard. Induction of alkaline phosphatase acrtivity in ATDC5 cells: EC50 = 40Nm. Endotoxin: Less than 0.1ng/ug (IEU/ug) of GDF-5. Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.15 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of GDF-5 as a Reference Standard. Storage and Stability: Lyophilized powder is stable for at least 12 months at -20°C Reconstituted product must be stored in aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 26.8kD |
Source: E. coli Purity: 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE Silver Stained gel. Endotoxin: 0.1ng/ug (IEU/ug). Form: Supplied as a lyophilized powder without any additives. Reconstitute with sterile 20mM AcOH to 100ug/ml, which can then be further diluted to other aqueous solutions with 0.1% BSA or HSA. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Spine 31(25): 2909-2917 (2006). 2. Connect Tissue Res. 47(5): 264-270 (2006). 3. J. Anat. 201(5): 425 (2002). 4. J. Anat. 200(2): 213 (2002). 5. J. Anat. 200(2): 213 (2002). 6. J. Anat. 200(2): 212 (2002).
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