Growth and Differentiation Factor 9 (GDF9)
|Growth Factors, Cytokines||Storage: -20°CShipping: Blue Ice|
Growth differentiation factor 9 (GDF9) is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. BMP proteins are secreted growth factors that are characterized by seven conserved cysteine residues. In general, they are regulators of cell growth and differentiation in both embryonic and adult tissues. GDF9 can form non-covalent homodimers when expressed individually, while when GDF9 and BMP15 are co-expressed BMP-15/GDF-9 heterodimers are produced. GDF9 signaling might be transduced by binding to the bone morphogenetic protein receptor (BMPR) type II and one or more BMPR type I receptors.
Recombinant human GDF-9 is a 31kD non-disulfide-linked homodimer containing two 135 amino acid polypeptide chains.
Endotoxin: 0.1ng/ug (1EU/ug).
GQETVSSELK KPLGPASFNL SEYFRQFLLP QNECELHDFR LSFSQLKWDN WIVAPHRYNP RYCKGDCPRA VGHRYGSPVH TMVQNIIYEK LDSSVPRPSC VPAKYSPLSV LTIEPDGSIA YKEYEDMIAT KCTCR
Suitable for use in ELISA, Western Blotting. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Storage & Stability:
The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted GDF-9 should be stored in working aliquots at -20°C.
Country of Origin:
Source: E. coli
Purity: 98% by SDS-PAGE and HPLC analyses.
Form: Supplied as a lyophilized powder.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Liao WX, Moore RK, Otsuka F, Shimasaki S: Effect of intracellular interactions on the processing and secretion of bone morphogenetic protein-15 (BMP-15) and growth and differentiation factor-9. Implication of the aberrant ovarian phenotype of BMP-15 mutant sheep. J. Biol. Chem. 278(6): 3713-3719 (2003).
Vitt UA, Mazerbourg S, Klein C, Hsueh AJ: Bone morphogenetic protein receptor type II is a receptor for growth differentiation factor-9. Biol. Reprod. 67(2): 473-480 (2002).
Aaltonen J, Laitinen MP, Vuojolainen K, Jaatinen R, Horelli-Kuitunen N, Seppa L, Louhio H, Tuuri T, Sjoberg J, Butzow R, Hovata O, Dale L, Ritvos O: Human growth differentiation factor 9 (GDF-9) and its novel homolog GDF-9B are expressed in oocytes during early folliculogenesis. J. Clin. Endocrinol. Metab. 84(8): 2744-2750 (1999).
Dong J, Albertini DF, Nishimori K, Kumar TR, Lu N, Matzuk MM: Growth differentiation factor-9 is required during early ovarian folliculogenesis. Nature 383(6600): 531-535 (1996).
McGrath SA, Esquela AF, Lee SJ: Oocyte-specific expression of growth/differentiation factor-9. Mol. Endocrinol. 9(1): 131-136 (1995).