Technical Data
G8990-10H
Growth Associated Protein 43 (GAP-43)
Description:
GAP-43 or growth associated protein 43 is a nervous system-specific phosphoprotein and a major component of motile growth cones which form the leading edge of the elongating axon. The membrane skeleton of the growth cone is responsible for transducing extracellular guidance cues into morphological alterations mediated by the polymerization/de-polymerization of actin resulting in directional changes in axon growth. GAP-43 is closely associated with the membrane skeleton and has been implicated in regulating interactions between cytoskeletal components and the plasma membrane. Interestingly, axonal transport of GAP-43 occurs only in neurons that are extending axons during development or regeneration after injury; however, this transport does not occur in axons that do not normally regenerate such as those in the mammalian central nervous system. These results suggest that the in vivo function of GAP-43 may be closely linked to axon growth and regeneration.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA (Immunogen): 0.1-1ug/ml
Western Blot: 1ug/ml
Immunohistochemistry (Frozen and paraffin): 5ug/ml
Optimal dilutions to be determined by the researcher.

WB Positive Controls:
Cell lysates derived from mouse and rat brain.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k3G162Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatMouse
Concentration:
~0.5mg/ml
Immunogen:
Puri ed, full length GAP-43 protein (both phosphorylated & unphosphorylated forms).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes rat GAP-43. Reacts with both phosphorylated and unphosphorylated forms of the GAP-43 protein. Species Crossreactivity: chicken, human, mouse, rabbit and snake.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Meiri, K.F., et al., J. Cell Biol. 112: 991-1005 (1991). 2. Karns, L.R., et al., Science 236: 597-600 (1987). 3. Basi, G.S., et al., Cell 49: 785-791 (1987). 4. Meiri, K.F., et al., Proc. Natl. Acad. Sci. 83: 3537-3541 (1986). 5. Tanaka, E., and Sabry, J., Cell 83: 171-176 (1995). 6. Meiri, K.F. and Gordon-Weeks, P.R., J. Neurosci. 10: 256-265 (1990). 7. Skene, J.H.P., Ann. Rev. Neurosci. 12: 127-156 (1989). 8. Coggins, P., and Zwiers, H., J. Neurochem. 53: 1895-1901 (1989). 9. Apel, E.D., et al., Biochemistry 29: 2330-2335 (1990). 10. Alexander, K., et al., J. Biol. Chem. 262: 6108-6113 (1987). 11. Chapman, E., et al., J. BIol. Chem. 266: 207-213 (1991). 12. Wang, K., and Roufogalis, B., Biochem. J. 262: 693-706 (1989). 13. Apel, E.D., Storm, D., Perspect. Dev. Neurobiol. 1: 3-11 (1992). 14. Dent, E.W. and Meiri, K.F., J. Neurobiol. 8: 1037-1053 (1992). 15. He, Q., et al., J. Neurosciences 17: 3515-3524 (1997). 16. Meiri, K.F., et al., J. Neurobiology 29: 213-232 (1995).