Technical Data
G9000-07
Growth Hormone, Human (hGH)
Description:
hGH is produced by the somatotroph cells in the pituitary. hGH acts through binding to membrane receptors that belong to the cytokine receptor superfamily. Ligand binding induces receptor dimerization and activation of the receptorassociated kinase: JAK2; this results in phosphorylation of the kinase itself, of the receptor, and of many cellular proteins including the Stat proteins as well as adaptors leading to the activation of the Ras/MAP kinase pathway and of the PI-3 kinase pathway.

Applications:
Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.

Recommended Dilutions:
Western Blot: 1-2ug/ml for 2 hours at RT.
Immunohistochemistry (Formalin/paraffin): 1:2000-4000 for 20 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilution to be determined by researcher.

Positive Control:
Recombinant human growth hormone. Human anterior pituitary

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k3F199Affinity Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Purified rcombinant human growth hormone. Cellular Localization: Cytoplasmic MW of Antigen: ~22kD.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See G9000-07X.
Specificity:
Recognizes human Growth Hormone (hGH).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Strasburger C , et al. (1989) Endocrinology, 124: 15481557. 2. Strasburger C, et al. (1989) J Clin Chem, 35: 15.