		Technical Data
H0529-18	3-hydroxybutyrate dehydrogenase, type 1 (3-hydroxybutyrate dehydrogenase, BDH, BDH1, D-beta-hydroxybutyrate dehydrogenase, mitochondrial, MGC2723, MGC4347, MGC9788, SDR9C1)

Description:
BDH1 (3-hydroxybutyrate dehydrogenase, type 1), it is a member of the short-chain dehydrogenase/reductase gene family. This protein forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. It catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000
Western Blot: 1:500-1:2000
Immunohistochemistry (Paraffin): 1:200-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	10F603	Ascites

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Mouse

Concentration:

Not determined

Immunogen:

Purified recombinant fragment of human 3-hydroxybutyrate dehydrogenase, type 1 expressed in E. coli.

Purity:

Ascites

Form

Supplied as liquid, 0.04% sodium azide.

Specificity:

Recognizes human 3-hydroxybutyrate dehydrogenase, type 1 at 38kD. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Biochemistry. 1996 Jun 25; 35(25):8158-65, 2. Biochemistry. 2000 Oct 3; 39(39):11928-38, 3. Proc Natl Acad Sci U S A. 2001 Dec 18; 98(26):15089-94.