Technical Data
H0529-18
3-hydroxybutyrate dehydrogenase, type 1 (3-hydroxybutyrate dehydrogenase, BDH, BDH1, D-beta-hydroxybutyrate dehydrogenase, mitochondrial, MGC2723, MGC4347, MGC9788, SDR9C1)
Description:
BDH1 (3-hydroxybutyrate dehydrogenase, type 1), it is a member of the short-chain dehydrogenase/reductase gene family. This protein forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. It catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000
Western Blot: 1:500-1:2000
Immunohistochemistry (Paraffin): 1:200-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG110F603Ascites
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Purified recombinant fragment of human 3-hydroxybutyrate dehydrogenase, type 1 expressed in E. coli.
Purity:
Ascites
Form
Supplied as liquid, 0.04% sodium azide.
Specificity:
Recognizes human 3-hydroxybutyrate dehydrogenase, type 1 at 38kD. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Biochemistry. 1996 Jun 25; 35(25):8158-65, 2. Biochemistry. 2000 Oct 3; 39(39):11928-38, 3. Proc Natl Acad Sci U S A. 2001 Dec 18; 98(26):15089-94.