Technical Data
H0529-20-P
3-Hydroxybutyrate Dehydrogenase, Type 2, aa92-103 (DHRS6, BDH2, EFA6R, FLJ13261, PRO20933, UCPA-OR, UNQ6308, dehydrogenase/reductase (SDR family) member 6, oxidoreductase UCPA (Control Peptide)
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
C-terminus of aa-DCEEKDWDFSMN
Control peptide corresponding to polyclonal antibody, Catalog#H0529-20, goat x human.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Peptide Blocking (see corresponding antibody H0529-20):
Antibodies are typically supplied at 0.5mg/ml and peptides as a 100ul pellet. When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Purity: Highly Purified
Concentration: ~0.5mg/ml
Form: Supplied as a lyophilized powder in PBS.
Specificity: Recognizes human BDH2. Species Crossreactivity: mouse, rat, bovine.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Characterization of human DHRS6, an orphan short chain dehydrogenase/reductase enzyme: a novel, cytosolic type 2 R-beta-hydroxybutyrate dehydrogenase., Guo K, Lukacik P, Papagrigoriou E, Meier M, Lee WH, Adamski J, Oppermann U., J Biol Chem. 2006 Apr 14;281(15):10291-7. Epub 2005 Dec 27.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.