Technical Data
H0529-30
4-Hydroxybutyrate CoA-transferase, Recombinant, Human, Western Blot Control (Gamma Hydroxybutyrate CoA Transferase)
10ug
Molecular Biology Storage: -20CShipping: Blue Ice
Clostridium aminobutyricum ferments 4-aminobutyrate to acetate and butyrate. One of the enzymes which catalyses this inter conversion of the intermediates is Gamma Hydroxybutyrate CoA Transferase. The aa sequence of the enzyme shows close relationships to proteins from the genomes of Clostridium difficile and Porphyromonas ginivalis. Characterisation of this enzyme from Clostridium aminobutyricum has shown it to be largely membrane bound, stable in anaerobic conditions.

Source:
Recombinant corresponding to aa1-438 from mature human 4-Hydroxybutyrate CoA-transferase, fused to 6x His-tag at N-terminal, expressed in E. coli.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: E. coli
Purity: Purified (SDS-PAGE)
Concentration: As reported
Form: Supplied as a liquid in 25mM Tris-HCl, pH6.8, 50mM DTT, 1% SDS, 0.1% Bromophenol Blue, 2.5% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Gerhardt, A. et al (2000) Arch.Microbiol. 174 (3): 189-199.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.