Technical Data
H1400
HA epitope, fusion tag
Description:
Expression of genes in E. coli or yeast or baculovirus offers a
convenient system to produce large amounts of recombinant
proteins that may otherwise be difficult to isolate from natural cells and tissues. Very often antibodies to these newly identified proteins are not available to study its biochemical properties, monitor protein expression, and purification. In order to circumvent this problem, short pieces of well-defined peptides (Poly-His, Flagepitope or c-myc epitope or HA-tag) or small proteins (bacterial GST, MBP, Thioredoxin, b-Galactosidase, VSV-Glycoprotein etc) are often cloned along with the target gene. Proteins are expressed as fusion proteins. Antibodies to these fusion-tags are already available to monitor fusion protein expression and purification. Therefore, fusion-tags serve as universal tags much like secondary antibodies.

Applications:
Suitable for use in ELISA, Immunohistochemistry, Immunofluorescenceand Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000-1:50,000. Using 50-100ng control antigen/well.
Western Blot: 1:1000-1:5000. Using chemiluminescence technque. Antibodies react with native and denatured his-tag containing proteins. 
Immunofluorescence: 10-50ug/ml
Immunohistochemistry (Frozen): 10-50ug/ml
Optimal dilutions to be determined by the researcher.

Recommended Control Peptide:
H1200-02: HA epitope, fusion tag, Control Peptide

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide containing the sequence YPYDVPDYA (aa114-122) derived from the influenza virus hemaglutinin (HA) protein (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as liquid in PBS, 0.1% BSA.
Specificity:
Recognizes recombinant proteins containing the HA epitope tag: YPYDVPDYA. Recognizes the HA-tag fused to either the N- or C-termini of targeted proteins. Species Crossreactivity: A broad species crossreactivity is expected based on conservation of sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Muster, T., et al. (1995) J. Virol. 69: 6678-6686.