Technical Data
Hanks’ Balanced Salts w/ Phenol Red, w/o CaCl2, MgSO4 (Powder)
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as g/liter
Phenol Red, Sodium0.0102
Potassium Chloride0.4
Potassium Phosphate Mono0.06
Sodium Chloride8
Sodium Phosphate Dibasic0.04788
Hanks' Balanced Salt Solution is designed for use with cells maintained in non-CO2 atmospheric conditions. Calcium and magnesium support cell adhesion. H1800 is also recommended to minimize cell attachment or aggregation. HBSS requires 0.35g/L of sodium bicarbonate and pH adjustment prior to filtration. It contain 1mg/L of glucose.

Directions per Liter: Dissolve 9.518grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Light yellow to orange, homogeneous, free flowing powder.

Solubility: Orange, clear, complete.

pH: As reported

Endotoxin: 1EU/ml

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Hanks, J.H., Wallace, R.E., PSEBM 71: 196 (1949).