Technical Data
Hanks’ Balanced Salts w/ NaHCO3 w/o CaCl2, MgSO4 (Powder)
Cell Culture Grade
Storage RT    Shipping RT
Components shown as g/liter
Inorganic Salts:
Calcium Chloride•2H2OAbsent
Magnesium SulfateAbsent
Potassium Chloride0.4
Potassium Phosphate Monobasic0.06
Sodium Chloride8.0
Sodium Phosphate Dibasic0.04788
Other Components:
Phenol Red, Sodium Salt0.0102
Sodium Bicarbonate0.3500
Hanks' Balanced Salt Solution is designed for use with cells maintained in non-CO2 atmospheric conditions. Calcium and magnesium support cell adhesion. H1800-10 is also recommended to minimize cell attachment or aggregation. HBSS requires pH adjustment prior to filtration. It contain 0.35g/L of sodium bicarbonate and 1g/L of glucose.

Directions per Liter Dissolve 9.9g in 800-900ml of ddH2O stirring gently until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L IFilter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains Sodium Bicarbonate.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Appearance: Light pink to pink, homogeneous, free flowing powder

Solubility: Light red to red, clear, complete

pH: 7.4 ± 0.6

Endotoxin: ~10EU/mg

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Hanks, J.H., Wallace, R.E., PSEBM 71: 196 (1949).