Technical Data
Media
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Technical Data
Media
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Hanks’ Balanced Salts w/ NaHCO3 w/o CaCl2, MgSO4 (Powder)
H1800-10
Cell Culture Grade
Storage RT Shipping RT
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Components shown as g/liter
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Hanks' Balanced Salt Solution is designed for use with cells maintained in non-CO2 atmospheric conditions. Calcium and magnesium support cell adhesion. H1800-10 is also recommended to minimize cell attachment or aggregation. HBSS requires pH adjustment prior to filtration. It contain 0.35g/L of sodium bicarbonate and 1g/L of glucose. Directions per Liter Dissolve 9.9g in 800-900ml of ddH2O stirring gently until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L IFilter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains Sodium Bicarbonate. Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time. Appearance: Light pink to pink, homogeneous, free flowing powder Solubility: Light red to red, clear, complete pH: 7.4 ± 0.6 Endotoxin: ~10EU/mg Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Hanks, J.H., Wallace, R.E., PSEBM 71: 196 (1949). |