Technical Data
Hanks’ Balanced Salts w/o CaCl2, MgSO4, Phenol Red (Powder)
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as g/liter
Potassium Chloride0.400
Potassium Phosphate Mono0.060
Sodium Chloride8.000
Sodium Phosphate Dibasic0.04788
Hanks' Balanced Salt Solution is designed for use with cells maintained in non-CO2 atmospheric conditions. Phenol red has been shown to interfere with the growth of some cells at cloning densities. Calcium and magnesium support cell adhesion. H1801 is also recommended to minimize cell attachment or aggregation. HBSS requires 0.35g/L of sodium bicarbonate and pH adjustment prior to filtration. It contain 1g/L of glucose.

Directions per Liter: Dissolve 9.508 grams in 800-900ml of ddH2O stirring gently until completely solubilized with heating. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Adjusting pH as necessary. If required, add sodium bicarbonate. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi)

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: White to off white, homogenous, free flowing powder

Solubility: Colorless, clear, complete

pH: 6.8 ± 0.4

Endotoxin: 1EU/ml

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Hanks, J.H., Wallace, R.E., PSEBM 71: 196 (1949).