Technical Data
Hanks’ Balanced Salts (10mg/L Phenol Red) (Powder)
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as mg/liter
Calcium Chloride•2H2O184.45
Magnesium Sulfate97.67
Potassium Chloride400
Potassium Phosphate Mono60
Sodium Chloride8000
Sodium Phosphate Dibasic47.88
Phenol Red, (Sodium)10.2
Hanks' Balanced Salt Solution is designed for use with cells maintained in non-CO2 atmospheric conditions. HBSS requires 0.35g/L of sodium bicarbonate and pH adjustment prior to filtration. It contains 1g/L of glucose.

Directions per Liter: Dissolve 9.8g in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. If required, add 0.35g/L sodium bicarbonate. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Pale orange to pink, homogenous, free flowing powder

Solubility: Yellow, clear, complete

pH As Reported:

Endotoxin: 1EU/ml

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Hanks, J.H., Wallace, R.E., PSEBM 71:196 (1949).