Technical Data
Media
Hanks Balanced Salts (10X) (Powder)
H1804
Cell Culture Grade
Storage RT    Shipping RT
Components shown as mg/liter
Calcium ChlorideĽ2H2O1855
Potassium Chloride4000
Potassium Phosphate Monobasic600
Sodium Chloride80000
Sodium Phosphate Dibasic475
Magnesium Sulfate977
D-Glucose10000
Phenol RedĽNa170
Total:98.1g/liter
Hanks' Balanced Salt Solution is designed for use with cells maintained in non-CO2 atmospheric conditions. HBSS requires 0.35g/L of sodium bicarbonate and pH adjustment prior to filtration. It contain 1g/L of glucose.


Directions per Liter: Dissolve 98.1 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. If required, add sodium bicarbonate. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121║C (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Appearance: Beige to orange, homogenous, free-flowing powder

Solubility (10X): Bright red, clear, complete; some precipitate may be observed due to excess concentration of salts. Sterile filter after solubilization.

Solubility (1X): Orange, clear, complete

pH (10X): 6.0 ▒ 0.4

pH (1X): 6.4 ▒ 0.4

Endotoxin: ~10EU/mg

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Hanks, J.H., Wallace, R.E., PSEBM 71: 196 (1949).