Technical Data
H1826-90
Hairy Cell Leukemia (HCL)
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Technical Data |
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H1826-90 |
Hairy Cell Leukemia (HCL) |
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Description: Hairy cell leukemia (HCL) is a chronic B-cell lymphoproliferative disorder characterized by pancytopenia and variable infiltration of the reticuloendothelial system with hairy lymphocytes. The lymphocytes present characteristic citoplasmatic projections and are positive for tartrate-resistant acid phosphatase (TRAP). It is more common in men than women and has a median age of diagnosis of 52yr. It is typically seen in middle-aged men, characterized by pancytopenia, splenomegaly, immunologic abnormalities, and morphologically typical neoplastic mononuclear cells in the blood, bone marrow, liver, spleen, and other tissues. In addition to pyogenic infections, patients are susceptible to unusual organisms including atypical mycobacterium, legionella, and fungi. Diagnosis for HCL is confirmed by demonstration of hairy cells in biopsy specimens from the bone marrow or spleen or in peripheral blood. Splenectomy is the standard initial treatment of choice. Recently, the nucleosides pentostatin (2'-deoxycoformycin) (DCF) and 2'-chlorodeoxyadenosine (2-CdA) have been shown to produce greater numbers of durable complete remissions with curative potential in patients with HCL. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (FFPE): 1:15-1:30. Incubate for 30 minutes at RT. Requires high temperature antigen unmasking with 10mM citrate buffer, pH 6.0 prior to immunostaining. Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Positive Control: Tonsil Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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