		Technical Data
H1827-28	HCN4 (Hyperpolarization-activated Cyclic Nucleotide-gated Potassium Channel 4)

Description:
Hyperpolarization-activated ion channel with very slow activation and inactivation exhibiting weak selectivity for potassium over sodium ions. May contribute to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP. May mediate responses to sour stimuli.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:200 using ECL on rat brain membranes.
Immunohistochemistry: mouse brain sections
Note: Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal dilutions to be determined by the researcher.

Control Antigen Included:
H1827-28A: HCN4, Control Antigen
Reconstitute with 100ul sterile PBS. For Positive Control in Western Blot, use 20ng of protein per Minigel lane. For negative control preincubate 3ug of fusion protein with 1ug antibody for 1hour at RT.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
50ul		-20°C	Blue Ice		Rabbit

Concentration:

~0.5mg/ml

Immunogen:

GST fusion protein corresponding to aa119-155 (HGHLH DSAEE RRLIA EGDAS PGEDR TPPGL AAEPE RP) of human HCN4 (Accession XP_007730, NP_005468, CAB42604, CAB52754)

Purity:

Purified by affinity chromatography.

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.05% sodium azide. Reconstitute with 50ul sterile ddH2O.

Specificity:

Recognizes HCN4. Species Crossreactivity: mouse, rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Han, W., et al. (2002) Circ. Res. 91, 790. 2. Doan, T.N., et al. (2004) J. Neurosci. 24, 3335. 3. Much, B., et al. (2003) J. Biol. Chem. 278, 43781.