Technical Data
H1827-55B
HDAC3 (Histone Deacetylase 3, HD3, RPD3, RPD3-2, SMAP45)
Description:
Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins (1). Eight members of HDAC family have been identified in the past several years (2,3). These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3). These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
This antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acids 2-17 of human HDAC3.
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity:
In HeLa, a 50kD band is observed. Species Crossreactivity: Crossreacts with Human. Expected to crossreact with Mouse (100% identity with immunogen), Rat (100% identity with immunogen) and Chicken (100% identity with immunogen) due to sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Meinke PT and Liberator P. Curr Med Chem 2001, 8(2):211-35.
2. Nakayama T and Takami Y. J Biochem (Tokyo) 2001, 129(4):491-9
3. Cress, W.D. and Seto, E. J. Cell. Physiol. 2000, 184, 1-16.