		Technical Data
H1827-55K2	HDAC4 (Histone Deacetylase 4, HA6116, HD4, HDACA, Histone Deacetylase A, KIAA0288)

Description:
Regulation of gene expression is mediated by several mechanisms, among them are DNA methylation, ATP-dependent chromatin remodeling, and posttranslational modifications of histones. These modifications include the dynamic acetylation and deacetylation of

Cellular Localization: Cytoplasmic and Nuclear

Positive Control: Total cell extracts of NIH3T3 fibroblast cells.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2a	8L116	Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Mouse

Concentration:

~2.2mg/ml

Immunogen:

Synthetic peptide: MSSQSHPDGLSGRDQPVEL, corresponding to amino acids 1-19 of Human HDAC4 conjugated to KLH with C-terminal added lysine.

Purity:

Purified by Protein A affinity chromatography.

Form

0.01M PBS [pH 7.4] containing 15 mM Sodium Azide

Specificity:

Species Crossreactivity: Crossreacts with Human, Mouse and Rat. Expected to crossreact with Chicken (100% identity with immunogen) due to sequence homology.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Fischle W et al. The emerging role of class II histone deacetylases. Biochem Cell Biol 79:337-48 (2001). Khochbin S et al. Functional significance of histone deacetylase diversity. Curr Opin Genet Dev 11:162-6 (2001). Wang AH et al. HDAC4, a human histone deacetylase related to yeast HDA1, is a transcriptional corepressor. Mol Cell Biol 19:7816-27 (1999). Grozinger CM et al. Three proteins define a class of human histone deacetylases related to yeast Hda1p. Proc Natl Acad Sci U S A 96:4868-73 (1999).