Technical Data
HDAC4 (Histone Deacetylase 4, HA6116, HD4, HDACA, Histone Deacetylase A, KIAA0288)
Regulation of gene expression is mediated by several mechanisms, among them are DNA methylation, ATP-dependent chromatin remodeling, and posttranslational modifications of histones. These modifications include the dynamic acetylation and deacetylation of

Cellular Localization: Cytoplasmic and Nuclear

Positive Control: Total cell extracts of NIH3T3 fibroblast cells.

Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG2a8L116Affinity Purified
100ul-20CBlue IceHumanMouse
Synthetic peptide: MSSQSHPDGLSGRDQPVEL, corresponding to amino acids 1-19 of Human HDAC4 conjugated to KLH with C-terminal added lysine.
Purified by Protein A affinity chromatography.
0.01M PBS [pH 7.4] containing 15 mM Sodium Azide
Species Crossreactivity: Crossreacts with Human, Mouse and Rat. Expected to crossreact with Chicken (100% identity with immunogen) due to sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Fischle W et al. The emerging role of class II histone deacetylases. Biochem Cell Biol 79:337-48 (2001). Khochbin S et al. Functional significance of histone deacetylase diversity. Curr Opin Genet Dev 11:162-6 (2001). Wang AH et al. HDAC4, a human histone deacetylase related to yeast HDA1, is a transcriptional corepressor. Mol Cell Biol 19:7816-27 (1999). Grozinger CM et al. Three proteins define a class of human histone deacetylases related to yeast Hda1p. Proc Natl Acad Sci U S A 96:4868-73 (1999).