Description:
Cellular Localization: Cytoplasmic and Nuclear
Positive Control: Whole extracts of rat brain, whole extracts of mouse NIH-3T3 cells (Western blotting) and HeLa nucle
Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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| Type | Isotype | Clone | Grade |
|---|
| Pab | IgG | | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 50ul | | -20°C | Blue Ice | Human | Rabbit |
|
| Concentration: |
| ~1mg/ml |
| Immunogen: |
| Synthetic peptide: DQPVELLNPARVNHM with C-terminal added lysine, conjugated to KLH, corresponding to amino acids 14-28 of Human HDAC4. The corresponding sequence is identical in mouse and differs by one amino acid in chicken. |
| Purity: |
| Purified by immunoaffinity chromatography. |
| Form |
| 15mM Sodium Azide; 1% BSA, 0.01M PBS. pH 7.4 |
| Specificity: |
| Species Crossreactivity: Crossreacts with Human, Mouse and Rat. Expected to crossreact with Chicken (93% identity with immunogen) due to sequence homology. Not yet tested in other species. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
Fischle W et al. The emerging role of class II histone deacetylases. Biochem Cell Biol 79:337-48 (2001). Khochbin S et al. Functional significance of histone deacetylase diversity. Curr Opin Genet Dev 11:162-6 (2001). Wang AH et al. HDAC4, a human histone deacetylase related to yeast HDA1, is a transcriptional corepressor. Mol Cell Biol 19:7816-27 (1999). Grozinger CM et al. Three proteins define a class of human histone deacetylases related to yeast Hda1p. Proc Natl Acad Sci U S A 96:4868-73 (1999).
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