Technical Data
HDAC4 (Histone Deacetylase 4, HA6116, HD4, HDACA, Histone Deacetylase A, KIAA0288)
Cellular Localization: Cytoplasmic and Nuclear

Positive Control: Whole extracts of rat brain, whole extracts of mouse NIH-3T3 cells (Western blotting) and HeLa nucle

Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
50ul-20CBlue IceHumanRabbit
Synthetic peptide: DQPVELLNPARVNHM with C-terminal added lysine, conjugated to KLH, corresponding to amino acids 14-28 of Human HDAC4. The corresponding sequence is identical in mouse and differs by one amino acid in chicken.
Purified by immunoaffinity chromatography.
15mM Sodium Azide; 1% BSA, 0.01M PBS. pH 7.4
Species Crossreactivity: Crossreacts with Human, Mouse and Rat. Expected to crossreact with Chicken (93% identity with immunogen) due to sequence homology. Not yet tested in other species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Fischle W et al. The emerging role of class II histone deacetylases. Biochem Cell Biol 79:337-48 (2001). Khochbin S et al. Functional significance of histone deacetylase diversity. Curr Opin Genet Dev 11:162-6 (2001). Wang AH et al. HDAC4, a human histone deacetylase related to yeast HDA1, is a transcriptional corepressor. Mol Cell Biol 19:7816-27 (1999). Grozinger CM et al. Three proteins define a class of human histone deacetylases related to yeast Hda1p. Proc Natl Acad Sci U S A 96:4868-73 (1999).