Cellular Localization: Cytoplasmic and Nuclear
Positive Control: Whole extracts of rat brain, whole extracts of mouse NIH-3T3 cells (Western blotting) and HeLa nucle
Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|Synthetic peptide: DQPVELLNPARVNHM with C-terminal added lysine, conjugated to KLH, corresponding to amino acids 14-28 of Human HDAC4. The corresponding sequence is identical in mouse and differs by one amino acid in chicken.|
|Purified by immunoaffinity chromatography.|
|15mM Sodium Azide; 1% BSA, 0.01M PBS. pH 7.4|
|Species Crossreactivity: Crossreacts with Human, Mouse and Rat. Expected to crossreact with Chicken (93% identity with immunogen) due to sequence homology. Not yet tested in other species.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
Fischle W et al. The emerging role of class II histone deacetylases. Biochem Cell Biol 79:337-48 (2001). <11467747>Khochbin S et al. Functional significance of histone deacetylase diversity. Curr Opin Genet Dev 11:162-6 (2001). <11250139>Wang AH et al. HDAC4, a human histone deacetylase related to yeast HDA1, is a transcriptional corepressor. Mol Cell Biol 19:7816-27 (1999). <10523670>Grozinger CM et al. Three proteins define a class of human histone deacetylases related to yeast Hda1p. Proc Natl Acad Sci U S A 96:4868-73 (1999). <10220385>|