Technical Data
H1827-56A
HDAC7 (Histone Deacetylase 7, DKFZP586J0917, HD7a)
Description:
Regulation of gene expression is mediated by several mechanisms. Among them are DNA methylation, ATP-dependent chromatin remodeling, and posttranslational modifications of histones, such as the dynamic acetylation and deacetylation of

Cellular Localization: Nuclear and cytoplasmic

Positive Control: Mouse NIH-3T3 cells (Western blotting). Normal rat kidney (NRK) cells (indirect immunofluorescence)

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to amino acids 357-373 of Human HDAC7 (RSEPLPPSATAPPPPGP) conjugated to KLH. The corresponding sequence is identical in mouse.
Purity:
Purified by immunoaffinity chromatography.
Form
15mM Sodium Azide. 1% BSA, 0.01M PBS. pH 7.4
Specificity:
Predicted molecular weight: 105kD. Additional bands may be detected in various extract preparations. Detection of HDAC7 is specifically inhibited with the immunizing peptide. Species Crossreactivity: Crossreacts with Human, Mouse and Rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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