Technical Data
HDAC7 (Histone Deacetylase 7, DKFZP586J0917, HD7a)
Regulation of gene expression is mediated by several mechanisms. Among them are DNA methylation, ATP-dependent chromatin remodeling, and posttranslational modifications of histones, such as the dynamic acetylation and deacetylation of

Cellular Localization: Nuclear and cytoplasmic

Positive Control: Mouse NIH-3T3 cells (Western blotting). Normal rat kidney (NRK) cells (indirect immunofluorescence)

Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
50ul-20CBlue IceHumanRabbit
Synthetic peptide corresponding to amino acids 357-373 of Human HDAC7 (RSEPLPPSATAPPPPGP) conjugated to KLH. The corresponding sequence is identical in mouse.
Purified by immunoaffinity chromatography.
15mM Sodium Azide. 1% BSA, 0.01M PBS. pH 7.4
Predicted molecular weight: 105kD. Additional bands may be detected in various extract preparations. Detection of HDAC7 is specifically inhibited with the immunizing peptide. Species Crossreactivity: Crossreacts with Human, Mouse and Rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wang, A.H., et al., Mol. Cell. Biol., 19, 7816-7827 (1999).
2. Grozinger, C.M., et al., Proc. Natl. Acad. Sci. USA, 96, 4868-4873 (1999).
3. Fischle, W., et al., Biochem. Cell Biol., 79, 337-348 (2001).
4. Khochbin, S., et al., Curr. Opin. Genet. Dev., 11, 162-166 (2001).
5. Fischle, W., et al., J. Biol. Chem., 274, 11713-11720 (1999).
6. Fischle, W., et al., J. Biol. Chem., 276, 35826-35835 (2001).
7. Dressel, U., J. Biol. Chem., 276, 17007-17013 (2001).
8. Kao, H.Y., et al., J. Biol. Chem., 276, 47496-47507 (2001).
9. Lemercier, C., et al., J. Biol. Chem., 277, 22045-22052 (2002).