		Technical Data
H1827-56D	HDAC7 (Histone Deacetylase 7, DKFZP586J0917, HD7a)

Description:
Human HDAC7 is composed of 912 amino acid residues. Although HDAC7 is localized mostly to the cell nucleus, it is also found in the cytoplasm, suggesting nucleo-cytoplasmic shuttling. The histone deacetylase activity of HDAC7 maps to a carboxy-terminal domain and is dependent on interaction with class I HDACs in the nucleus. HDAC7 catalyzes removal of acetyl-groups from acetyl-lysines of histones and promotes compaction of chromatin in these regions, leading to the inhibition of gene transcription.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-4ug/ml
Immunohistochemistry: 20-40ug/ml
Immunoprecipitation: 20-40ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Mouse	Rabbit

Concentration:

~0.2mg/ml

Immunogen:

Synthetic peptide surrounding amino acid 921 of murine HDAC7 (ID# BV-79)

Purity:

Purified by Immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.01% thimerosal, 30% glycerol.

Specificity:

Recognizes mouse HDAC7 at ~105kD. Species Crossreactivity: human and rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.