Technical Data
H1827-56F6
HDAC8, phosphorylated (Ser39) (Histone Deacetylase 8, HD8, CDA07, HDAC8, HDACL1)
Description:
HDAC8 is a 377 residue class I enzyme that lies close to the phylogenetic boundary between the class I and class II histone deacetylases and maps to the X chromosome (Xq13). HDAC8, under the regulation of PKA, plays an important role in determining the acetylation state of histones. HDAC8 is phosphorylated by cyclic AMP-dependent protein kinase A (PKA) which decreases the activity of HDAC8 and leads to the hyperacetylation of histones H3 and H4. Pathological role of HDAC8 has been observed in one of the most frequent types of acute myeloid leukemia (AML).

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic phosphopeptide corresponding to human HDAC8 around the phosphorylation site of serine 39 (R-A-SP-M-V).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes human HDAC-8, phosphorylated at Ser39. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. John, R. et al. Structure. 12:1325-1334 (2004).