		Technical Data
H1827-57E	HDAC11 (Histone Deacetylase 11, HD11, HD-11, FLJ22237)

Description:
HDAC11 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation is a marker for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. The predominantly nuclear HDAC11, which interacts with HDAC6, is weakly expressed in most tissues, and strongly expressed in brain, heart, skeletal muscle, kidney and testis. Its activity is inhibited by trapoxin, a known histone deacetylase inhibitor.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000
Western Blot: 1:25
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Positive Control:
Mouse Brain cell lysate

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic peptide corresponding the N-terminal region of human HDAC11 (KLH).

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid, 0.09% sodium azide.

Specificity:

Recognizes human HDAC11. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Voelter-Mahlknecht S., et al. (2005). Int J Mol Med. 16(4):589-98. 2. Bradbury CA., et al. (2005). Leukemia. 19(10):1751-9. 3. Gregoretti IV., et al. (2004). J Mol Biol. 338(1):17-31. 4. Gao, L., et al. (2002). J. Biol. Chem. 277(28):25748-25755.