		Technical Data
H1827-66C	HDAC6 (Histone Deacetylase 6, FLJ16239, HD6, JM21, KIAA0901)

Description:
Human HDAC6 (1215aa residues) possesses two separate putative catalytic domains, both of which are fully functional and contribute independently to the overall activity of HDAC6. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. HDAC6 may participate in coordinating expression of a group of genes involved in the remoldeling of chromatin during cell differentiation.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-4ug/ml
Immunohistochemistry: 20ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Mouse	Rabbit

Concentration:

~0.2mg/ml

Immunogen:

Synthetic peptide corresponding to the N-terminus of mouse HDAC-6.

Purity:

Purified by affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.01% thimerosal, 30% glycerol.

Specificity:

Recognizes mouse HDAC-6 at ~134kD. Does not cross react with other HDAC proteins including HDAC1, 2, 3, 4, 5, 7 and 8. Species Crossreactivity: human and rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.