Technical Data
Media
Heart Infusion Broth (Powder)
H1829-05
Microbiological Grade
Storage RT    Shipping RT
Components shown as g/Liter:
Beef Heart Infusion 2.0
Pancreatic Digest of Casein10.0
Peptic Digest of Animal Tissue 5.0
Sodium Chloride 5.0
Yeast Extract 3.0
Total: 25g/Liter
General purpose nutritive medium.

Heart Infusion Broth medium is prepared from an infusion of beef heart. The first media used for the cultivation of bacteria was prepared from meat infusions. Huntoon showed that pathogenic microorganisms could be grown from infusion agar without enrichments.

Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.


Directions: Dissolve 25g in 800-900ml of ddH2O stirring gently until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability: Store powdered media at 4C. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be stored at 4C and used within a short period of time.

Microbiological Analysis: Escherichia coli ATCC 25922

Appearance: Light beige, homogeneous, free flowing powder.

Solubility: Light to medium amber, clear, complete.

pH (2.5%): 7.40.2


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

US Biological application reference: Bubeck, S.S. et al., (2007) Infection and Immunity 75:697-670. 1. J. Infect. Dis. 23:169 (1918)