Technical Data
Heat Shock Protein 90 (HSP90)
HSP70 and HSP90 are molecular chaperones expressed constitutively under normal conditions to maintain protein homeostasis and induced upon environmental stress. Both HSP70 and HSP90 are able to interact with unfolded proteins to prevent irreversible aggregation and catalyze the refolding of the substrates in an ATP and co-chaperone dependent manner. HSP70 has broad range of substrates including newly synthesized and denatured proteins, while HSP90 tends to have a more limited subset of substrates, most of which are signaling molecules. HSP70 and HSP90 often function collaboratively in a multi-chaperone system, which requires a minimal set of co-chaperones: HSP40, Hop and p23. The co-chaperones either regulate the intrinsic ATPase activity of the chaperones or recruit chaperones to specific substrates or subcellular compartments. When the ubiquitin ligase CHIP associates with the HSP70/HSP90 complex as a cofactor, the unfolded substrates are subjected to degradation by the proteosome. The biological functions of HSP70/HSP90 go beyond their chaperone activity. They are essential for the maturation and inactivation of nuclear hormones and other signaling molecules. They also play a role in vesicle formation and protein trafficking.

Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000 detects a band at ~92kD.
Immunohistochemistry (Paraffin):1:100. Antigen retrieval: heating in citrate buffer.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptides corresponding to human HSP90 (KLH).
Purified by Protein A and peptide affinity chromatography.
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Recognizes endogenous levels of human total HSP90 protein, alpha and beta isoforms at ~92kD. Does not crossreact with other HSPs. Species Crossreactivity: mouse, rat, monkey and Drosophila. Species sequence homology: chicken, zebra fish and bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Nollen, E.A. and Morimoto, R.I. 2002. J. Cell Sci. 115, 28092816. 2. Young, J.C. et al. 2003. Trends Biochem. Sci. 28, 541547. 3. Pratt, W.B. and Toft, D.O. 2003. Exp. Biol. Med. 228, 111133. 4. Hohfeld, J. et al. 2001. EMBO Rep. 2, 885890.