Technical Data
H1835-25
HEC1
Description:
Human HEC1 is a coiled-coil protein that has been demonstrated to have a role in M-phase progression. It is essential for proper chromosome segregation. Its depletion results in the impairment of chromosome congression and results in spindle checkpoint activation.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:2000 (ECL)
Immunofluorescence: 4-10ug/ml
Immunoprecipitation: 1-5ug
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa whole cell lysates

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b,k4i287Affinity Purified
SizeStorageShippingSourceHost
100ul4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant protein encoding aa56-642 of human HEC1 purified from E. coli.
Purity:
Purified by Protein G affinity chromatography. 95% by SDS-PAGE.
Form
Supplied as a liquid in PBS, pH 7.4.
Specificity:
Recognizes human HEC1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Chen, Y., et al., Mol. Cell Bio. 17: 6049-6056 (1997). 2. Zheng, L., et al., Mol. Cell Bio. 20: 3529-3537 (2000). 3. Martin-Lluesma, S., et al., Science 297: 2267-2270 (2002). 4. Ciferri, C., et al., J. Biol. Chem. 280: 29,088-29,095 (2005).