Technical Data
H1847-51
Hemagglutinin Tag (YPYDVPDYA) (HA Tag)
Description:
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:5000
Immunohistochemistry: 1:500
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1906-10: IgY, Chicken (HRP) Rb x Ch
I1906-14: IgY, Chicken (AP) Rb x Ch

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
TypeIsotypeCloneGrade
PabIgYAffinity Purified
SizeStorageShippingSourceHost
1ml-20°CBlue IceChicken
Concentration:
~0.1mg/ml
Immunogen:
Synthetic peptide corresponding to the sequence YPYDVPDYA derived from the influenza virus hemaglutinin (HA) (KLH).
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity:
Recognizes recombinant proteins containing the HA epitope tag: YPYDVPDYA.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Terpe, K., Appl. Microbiol. Biotechnol. 60: 523-533 (2003). 2. Tu, Y., et al., Mol. Biol. Cell 9: 3367-3382 (1998). 3. Bhattacharya and Cabral, Mol. Biol. Cell 15(7): 3123-3131 (2004). 4. Yamanaka, et al., Curr. Biol. 13(9): 734-743 (2003). 5. Chang, et al., Genes Dev. 18(16): 2010-2023 (2004). 6. Munro, S., Pelham, H.R., EMBO J. 3: 3087-3093 (1984). 7. Madeo, F., et al., Mol. Cell 9: 911-917 (2002). 8. Ruckdeschel, K., et al., J. Immunol. 168: 4601-4611 (2002). 9. Field, J., et al., Mol. Cell. Biol. 8: 2159-2165 (1988). 10. Schirmer, E., et al., J. Cell Biol. 153: 479-490 (2001). 11. Bjerling, P., et al., Mol. Cell. Biol. 8: 2170-2181 (2002). 12. Wilson, l.A., et al., Cell 37: 767-78 (1984). 13. Muster, T., et al., J. Virol. 69: 6678-6686 (1995).