Technical Data
H1850-01B
Hemoglobin
Description:
Hemoglobin is composed of one globin plus four hemes; heme consists of protoporphyrin IX and ferrous iron. The iron content of human hemoglobin is 0.338% (w/w) which gives a minimal molecular weight of 16,520; however, ultracentrifugal and osmotic pressure measurements indicate an actual molecular weight four times the minimum molecular weight.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Capture ELISA: 5ug/ml
Western Blot: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceHumanGoat
Concentration:
As reported
Immunogen:
Human Hemoglobin.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.095% sodium azide.
Specificity:
Recognizes human Hemoglobin. Forms a single precipitin band with human adult hemoglobin A1, A2, F and S, by immunoelectrophoresis.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.