Technical Data
H1850-08D
Hemoglobin (HRP)
Description:
Hemoglobin is involved in oxygen transport from the lung to the various peripheral tissues.The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult Hemoglobin. The normal adult Hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta zero thalassemia. Reduced amounts of detectable beta globin causes beta plus thalassemia.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:10,000-1:100,000
Western Blot: 1:100-1:30,000
Immunohistochemistry: Paraffin sections
Immunocytochemistry: 1:200-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg-20°CBlue IceGoat
Concentration:
~1mg/ml
Immunogen:
Hemoglobin A1, A2, F and S
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.2% BSA, 0.1% Pro-Clean 400. Labeled with Horseradish Peroxidase (HRP).
Specificity:
Recognizes Hemoglobin. Reacts specifically with human Hemoglobin A1, A2, F and S. No reaction with human serum proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.