Technical Data
Media
Hemoglobin Powder, Bovine (Powder)
H1850-30
Microbiological Grade
Storage RT/4C    Shipping Blue Ice

Hemoglobin powder is freeze-dried hemoglobin for use in preparing microbiological culture media. Hemoglobin Powder is obtained from desiccated beef blood. Hemoglobin Powder is used with GC Agar in the preparation of Chocolate Agar, Thayer-Martin Medium, Modified Thayer-Martin Medium, Martin-Lewis Agar, and Transgrow Agar. Supplemented with Hemoglobin Powder and growth enrichment, these enriched media are used for the isolation and cultivation of fastidious microorganisms, especially Nesseria spp. and Haemophilus spp. Hemoglobin Powder provides hemin (X factor) required for growth of Haemophilus and for enhanced growth of Neisseria spp. In the preparation of Chocolate Agar, Hemoglobin powder is used in a 2% solution.

Hemoglobin is composed of one globin plus four hemes; heme consists of protoporphyrin IX and ferrous iron. The iron content of human hemoglobin is 0.338% (w/w) which gives a minimal molecular weight of 16,520; however, ultracentrifugal and osmotic pressure
measurements indicate an actual molecular weight four times the minimum molecular weight.

Application: Used to prepare chocolate agar, Thayer-Martin Medium, Modified Thayer-Martin Medium, Martin-Lewis Agar, and Transgrow Agar.

Growth:
Neisseira meningitidis ATCC 13090: Good growth
Haemophilus influenzae ATCC 10211: Good growth

Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Country of Origin: USA


Source: Bovine blood Appearance


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.