Technical Data
H1890
Heparan Sulfate (10E4 epitope)
Description:
Heparan sulfate, otherwise known as heparitin sulfate or heparin monosulfate, is a generic term describing polysaccharides which are linear and consist of N-acetylated [->4)alpha-D-GlcNpAc-(1->4) -D-GlcAp(1->] and N-sulfated disaccharides [->4)alpha-D-GlcNpS- (1->4)--D-GlcAp or alpha-L-IdoAp(1->] that are arranged mainly in a segregated manner. The sulfate-rich fractions in heparan sulfate are heparin-like, though they rarely possess the sulfate density found in heparin. Approximately 25% of the total polymer is initially formed by alternating arrangements of the two disaccharide units, ->4)alpha-D-GlcNpS(1->4)UAp(1->4) alpha-D-GlcNpAc(1->4)UAp (1->4)-alpha-D-GlcAp(1->. The polymer is formed as a repeating ->4)-alpha-D-GlcNpAc (1->4)--D-GlcAp(1-> disaccharide sequence that is attached to a serine residue of a core protein through a tetrasaccharide, glucuronosyl->galactosyl->galactosyl->xylosyl, linkage region. It then undergoes partial N-deacetylation followed by N-sulfation of the newly exposed amino groups, partial C-5 epimerization of D-GlcAp to L-IdoAp and O-sulfation. O-sulfates are always found in proximity to N-sulfates which enhances the clustering of the sulfate residues and the heterogeneity in chemical composition and charge density of heparan sulfate.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, ELISA and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Flow Cytometry: 1:70-1:140 (0.5-1ug labels ~10e5 cells)
Immunohistochemistry: 1:35-1:70
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgM,k8.S.087Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Liposome-incorporated membrane heparan sulfate proteoglycan from human fetal lung fibroblasts.
Purity:
Purified by thiophilic affinity chromatography using Prosep Thiosorb M.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Specificity:
Recognizes an epitope present in many types of human heparan sulfate. The epitope includes N-sulfated glucosamine residues that are critical for the reactivity of the antibody. Does not react with hyaluronan, chondroitin sulfate, dermatan sulfate, keratan sulfate or DNA. Reactivity with most heparan sulfates is nearly completely abolished after treatment of the glycosaminoglycan with bacterial heparitinase (Flavobacterium heparinum, EC 4.2.2.8).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application references: 1. Geelen, J. et al., (2008) Nephrology Dialysis Transplantation 23:3091-3095. 2. Bacsa, S. et al., (2010) doi:10.1099/vir.0.027052-0. 3. Clment, A. et al, (2008) PLoS Genet 4: e1000136. doi:10.1371/journal.pgen.1000136. General References: 1. David, G., et al., J. Cell Biol. 119: 961-975 (1992). 2.Bai, X.M., et al., J. Histochem. Cytochem. 42: 1043-1054 (1994). 3. Lories, V., et al., J. Biol. Chem. 264: 7009-7016 (1989). 4. Fuxe, K., et al., Brain Res. 636: 131-138 (1994). 5. Nackaerts, K., et al., Int. J. Cancer (Pred. Oncol.) 74: 335-345 (1997). 6. van Kuppevelt, T., et al., J. Biol. Chem. 273: 12,960-12,966 (1998). 7. Yokoyama, H., et al., Kidney Int. 56: 650-658 (1999). 8. Oshiro, M., et al., Histochem. Cell Biol. 115: 373-380 (2001). 9. van den Born, J., et al., J. Biol. Chem. 280: 20,516-20,523 (2005).