Technical Data
H1890-90F
Heparan Sulfate Proteoglycan (Sdc2, Syndecan 2, Fibroglycan, Fibroglycan, Heparan sulfate proteoglycan core protein, HSPG, Hspg1, Synd2, SYND2)
Description:
Proteoglycans are macromolecules consisting of a variety of core proteins with covalently attached one or several polysaccharide chains of the glycosaminoglycan type (heparan sulfate, heparin, chondroitin sulfate, dermatan sulfate or keratan sulfate). At least two forms of basement membrane heparan sulfate proteoglycan (HSPG) have been identified. One with a large core protein (> 400kD) and one with a small core protein (30kD). The large HSPG is probably the most abundant basement membrane proteoglycan. It is located predominantly in the lamina lucida, where it forms clustered aggregates and interacts with other basement membrane components to form the matrix. In addition, it also plays a critical role in attachment of cells to the basal membrane via integrin receptors.

Applications:
Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 110-1:500
Immunohistochemistry (Frozen): 1-2ug/ml. Can also be used with paraffin sections.
Note: Requires unmasking with high or low pH buffer reveals different aspects of heparan sulfate expression.
Optimal dilutions to be determined by the researcher.

Positive Control:
Tonsil

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a8L159Affinity Purified
SizeStorageShippingSourceHost
500ul4C Do not freezeBlue IceMouseRat
Concentration:
~0.2mg/ml
Immunogen:
Murine EHS laminin preparation. Cellular Localization: Basement membrane.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH7.4, 0.2% BSA, 0.09% sodium azide.
Specificity:
Recognizes mouse domain IV of the core protein of the large heparan sulfate proteoglycan. The reactivity is independent of the galactosaminoglycan moieties. Therefore, the epitope is not sensitive to heparitinase treatment. Species Crossreactivity: Human, porcine, bovine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Couchman et. al., Matrix, 1989; 9:311-321. 2. Horiguchi et. al., J Histochem Cytochem, 1989; 37:961-970. 3. Ljubimov et. al., Int J Cancer, 1992; 50:562-566. 4. Guelstein et. al., Int J Cancer, 1993; 53:269-277. 5. Ljubimov et. al., Lab Invest, 1995; 72:461-473.