Technical Data
H1890-92
Heparan Sulfate Proteoglycan, Domain 4 (Perlecan, HSPG)
Description:
Perlecan is a major heparan sulfate proteoglycan (HSPG) found within all basement membranes and cell surfaces. Because of its strategic location and ability to store and protect growth facors, perlecan hs been stronglyimplicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth promoting attributes primarily by acting as a coreceptor for basic fibroblast growth factor. Suppression of perlican causes substantial inhibition of neoplastic growth and neovasacularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogaenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.

Applications:
Suitable for use in Immunofluorescence and Immunohistochemistry. Not suitable for use in Western Blot. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (Frozen and acid-alcohol fixed, paraffin embedded sections): 1-2ug/ml for 30 minutes at RT. Staining of acid-alcohol-fixed tissues requires antigen unmasking with 15,000u/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 minutes at 37C
Optimal dilutions to be determined by the researcher.

Positive Control:
Tonsil

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k0.N.325 (A7L6)Affinity Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceMouseRat
Concentration:
~0.2mg/ml
Immunogen:
Mouse EHS laminin preparation. Cellular Localization: Basement membrane
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See H1890-92X.
Specificity:
Recognizes mouse Heparan Sulfate Proteoglycan. Does not crossreact with laminin, fibronectin or dermatran sulfate proteoglycan. Species Crossreactivity: human, bovine and porcine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
11. Folkvord et. al., J Histochem Cytochem, 1989; 37:105-113. 2. Couchman et. al., Matrix, 1989; 9:311-321. 3. Horiguchi et. al., J Histochem Cytochem, 1989; 37:961-970. 4. Ljubimov et. al., Int J Cancer, 1992; 50:562-566. 5. Guelstein et. al., Int J Cancer, 1993; 53:269-277. 6. Ljubimov et. al., Lab Invest, 1995; 72:461-473.