Technical Data
Heparan Sulfate Proteoglycan (Perlecan, HSPG)
Human perlecan (HSPG2) core protein is a large, complex, multidomain heparan sulfate proteoglycan with a MW of ~467kD (determined from the cDNA sequence). Post-translational modifications of HSPG2 can increase the MW to ~850kD. Perlecan is comprised of five domains. Domain I (N terminal) is predicted to be the site of heparan sulfate linkage to 3 glycosaminoglycans. Domain II is homologous to the LDL receptor. Domain III shares homology with the short arm of laminin A and B chains. Domain IV contains IgG-like repeats similar to those found in N-CAM and has a hydrophobic region suitable for interacting with the membrane. Domain V has regions similar to EGF and the G-domain of laminin A chain. In skin, perlecan is synthesized exclusively by the connective tissue in the dermal layer. Perlecan has been detected in many human tissues, including; breast, colon, liver, lymph nodes, pancreas, and prostate, among others. Proteins similar to perlecan have been identified in colon carcinoma, EHS tumor, and fibrosarcoma cells.

Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Positive Control:
Human breast, colon, liver, lymph nodes, pancreas and prostate tissues.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.    
100ug-20°CBlue IceHumanMouse
Recombinant protein corresponding to Perlecan domain III.
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Recognizes domain III of human Heparan Sulfate Proteoglycan. Domain III shares homology with the short arm of laminin A and B chains.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Murdoch, A.D., et al., J. Histochem. and Cytochem. 42(2): 239-249 (1994). 2. Murdoch, A.D., et al., J. Biol. Chem. 267: 8,544-8,557 (1992). 3. Cohen, I.R., et al., PNAS, USA 90:10,404-10,408 (1993). 4. Iozzo, R.V., J. Cell Biol. 99: 403-417 (1984). 5. Paulsson, M., et al., J. Mol. Biol. 197: 297-313 (1987). 6. Kallunki, P., et al., Genomics 11: 389-396 (1991).