Technical Data
H1890-93
Heparan Sulfate Proteoglycan (Perlecan, HSPG)
Description:
Human perlecan (HSPG2) core protein is a large, complex, multidomain heparan sulfate proteoglycan with a MW of ~467kD (determined from the cDNA sequence). Post-translational modifications of HSPG2 can increase the MW to ~850kD. Perlecan is comprised of five domains. Domain I (N terminal) is predicted to be the site of heparan sulfate linkage to 3 glycosaminoglycans. Domain II is homologous to the LDL receptor. Domain III shares homology with the short arm of laminin A and B chains. Domain IV contains IgG-like repeats similar to those found in N-CAM and has a hydrophobic region suitable for interacting with the membrane. Domain V has regions similar to EGF and the G-domain of laminin A chain. In skin, perlecan is synthesized exclusively by the connective tissue in the dermal layer. Perlecan has been detected in many human tissues, including; breast, colon, liver, lymph nodes, pancreas, and prostate, among others. Proteins similar to perlecan have been identified in colon carcinoma, EHS tumor, and fibrosarcoma cells.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-2ug/ml
Immunohistochemistry (Frozen or FFPE): 5-10ug/ml. Note: Formalin-fixed, paraffin sections require pretreatment with 0.1% protease for 30 minutes at RT prior to the blocking step.
Optimal dilutions to be determined by the researcher.

Positive Control:
Human breast, colon, liver, lymph nodes, pancreas and prostate tissues.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.    
TypeIsotypeCloneGrade
MabIgG13G166Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Recombinant protein corresponding to Perlecan domain III.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes domain III of human Heparan Sulfate Proteoglycan. Domain III shares homology with the short arm of laminin A and B chains.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Murdoch, A.D., et al., J. Histochem. and Cytochem. 42(2): 239-249 (1994). 2. Murdoch, A.D., et al., J. Biol. Chem. 267: 8,544-8,557 (1992). 3. Cohen, I.R., et al., PNAS, USA 90:10,404-10,408 (1993). 4. Iozzo, R.V., J. Cell Biol. 99: 403-417 (1984). 5. Paulsson, M., et al., J. Mol. Biol. 197: 297-313 (1987). 6. Kallunki, P., et al., Genomics 11: 389-396 (1991).