Technical Data
H1890-93A
Heparan Sulfate Proteoglycan (HSPG)
Description:
The principal molecular structure of basement membranes has been elucidated during the past two decades and it was shown that for example in the glomerular basement membrane (GBM) HS-PG are responsible for the selective filtration process. Removal of HS-PG will lead to proteinuria. Whereas previously perlecan was the only known basement membrane HS-PG, there is now evidence that (at least two) other basement membrane HS-PG do exist: Agrin, originally discovered as an important component of the neuromuscular junction and a novel small HS-PG, that was isolated from human aorta and kidney. This HS-PG, with a molecular weight of 80-200kD (aorta) and 30-160kD (kidney) and a core protein size of 24kD or 22kD, respectively, was localized by immunohisto-hemistry to the basement membrane. Amino acid sequence analysis of tryptic peptides indicate, that this small HS-PG is clearly distinct from perlecan and agrin.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: < 1ug/ml
Western Blot: 1ug/ml
Immunohistochemistry: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
TypeIsotypeCloneGrade
MabIgG1,k1F10/B8Affinity Purified
SizeStorageShippingSourceHost
10ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Purified human small basement membrane heparan sulfate proteoglycan (HS-PG)
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from 50mM Tris, pH 7.4. Reconstitute with 10ul sterile ddH2O.
Specificity:
Recognizes human Heparan-Sulfate-Proteoglycan. Specific for a core protein epitope of a human small basement membrane heparan sulfate proteo-glycan (HS-PG).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Stöcker G, Meyer HE, Wagener C and Greiling H (1991) Biochem J 274: 415-420 2. Heintz B, Stöcker G, Rentz U, Melzer H, Mrowka C, Stickeler E, Sieberth HG, Greiling H, Haubeck HD (1995) Hypertension 25: 399-407. 3. Stefanidis I, Heintz B, Stöcker G, Mrowka C, Sieberth HG, Haubeck HD (1996) J Am Soc Nephrol 7: 1-7. 4. Stöcker G, Stickeler E, Switalla S, Fischer DC, Greiling H, Haubeck HD (1997) Eur J Clin Chem Clin Biochem 35: 95-99