Technical Data
H1890-95A
Heparanase 1 (HEP1)
Description:
Heparanase is Heparanase is an endo-beta-D-glucuronidase, which degrades heparan sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix. Heparanase plays an important role in ECM degradation, facilitating the migration and extravasation of tumor cells and inflammatory leukocytes. Upon degradation, heparanase releases growth factors and cytokines that stimulate cell proliferation and chemotaxis. Heparanase is a heterodimer comprised of a 50kD subunit harboring the active site and a 8kD subunit. It is produced as a latent 65kD precursor and proteolytically processed to its active form. Heparanase is highly expressed in myeloid leukocytes (i.e. neutrophils) in platelets and in human placenta. Human heparanase was found to be upregulated in various types of primary tumors, correlating in some cases with increased tumor invasiveness and vascularity and with poor prospective survival.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:2000
Immunohistochemistry (Paraffin sections and blood smears): 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.n endo-beta-D-glucuronidase, which degrades heparan sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix. Heparanase plays an important role in ECM degradation, facilitating the migration and extravasation of tumor cells and inflammatory leukocytes. Upon degradation, heparanase releases growth factors and cytokines that stimulate cell proliferation and chemotaxis. Heparanase is a heterodimer comprised of a 50kD subunit harboring the active site and a 8kD subunit. It is produced as a latent 65kD precursor and proteolytically processed to its active form. Heparanase is highly expressed in myeloid leukocytes (i.e. neutrophils) in platelets and in human placenta. Human heparanase was found to be upregulated in various types of primary tumors, correlating in some cases with increased tumor invasiveness and vascularity and with poor prospective survival.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:2000
Immunohistochemistry (Paraffin sections and blood smears): 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
290ug-20°CBlue IceHumanRabbit
Concentration:
~2mg/ml
Immunogen:
50kD-8kD Heparanase heterodimer
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.01% thimerosal.
Specificity:
Recognizes the 65kD precursor as well as the 50kD and 8kD subunits of human Heparanase. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Vlodavsky, I., et al., Nat. Med. 5: 793-802 (1999). 2. Vlodavsky, I., et al., J. Clin. Invest. 108: 341-347 (2001). 3. Parish, C.R., et al., Biochem. Biophys. Acta 1471: M99-M108 (2001). 4. Vlodavsky, I., et al., Cancer Metastasis Rev. 9: 203-226 (1990). 5. Bashkin, P., et al., Biochemistry 28: 1737-1743 (1989). 6. Fairbanks, M.B., et al., J. Biol. Chem. 274: 29,587-29,590 (1999). 7. Koliopanos, A., et al., Cancer Res. 61: 4655-4659 (2001). 8. Gohji, K., et al., Int. J. Cancer 95: 295-301 (2001).